Fig. 3.

Line graphs illustrate the concentration-dependent effects of 5′-AMP (A), 3′-AMP (C), and 2′-AMP (E) on levels of adenosine in the medium of cultures from human AVSMCs. Bar graphs illustrate the effects of α,β-methylene-adenosine-5′-diphosphate [AMPCP; 100 μmol/l; ecto-5′-nucleotidase (CD73) inhibitor] on adenosine levels in the medium of human AVSMCs incubated with 10 μmol/l of either 5′-AMP (B), 3′-AMP (D), or 2′-AMP (F). P values are from Kruskal-Wallis one-way ANOVA on ranks. ^aP < 0.05 (Kruskal-Wallis multiple-comparison Z-value test) compared with basal (0 or PBS) or AMPCP. ^bP < 0.05 (Kruskal-Wallis multiple-comparison Z-value test) compared with 5′-AMP in the absence of AMPCP.