Fig. 4.
The receptor expressions of P2Y2, P2Y4, and P2Y6 are similar between RA from DOCA-salt and Uni rats. Analysis of P2Y2 (A), P2Y4 (B), and P2Y6 (C) protein expression in RA obtained from DOCA-salt and Uni rats is shown. Left: representative Western blots for P2Y2, P2Y4, P2Y6, and β-actin. Proteins were subjected to 10% SDS-PAGE and then transferred to nitrocellulose membranes. They were then incubated with a primary antibody specific for P2Y2 (42 kDa), P2Y4 (78 kDa), P2Y6 (100 kDa), or β-actin (42 kDa) and also with a secondary anti-rabbit or anti-mouse antibody. Right: bands were quantified as described in materials and methods. Ratios were calculated for the optical density of each receptor over that of β-actin. Data are means ± SE from 4 experiments.