Table 1.

List of primer sequences for quantitative PCR, the annealing temperature for each primer, and where the primers were obtained

Target Gene	Sequence	AT	Source
β-Actin	FP: 5′-ATCGCTGACAGGATGCAGAAG-3′	58	Zhu and Altmann (77)
	RP: 5′-TCAGGAGGAGCAATGATCTTGA-3′
Collagen I	FP: 5′-CCCAAGGAAAAGAAGCACGTC-3′	55	Li et al. (26)
	RP: 5′-AGGTCAGCTGGATAGCGACATC-3′
Collagen I	FP: 5′-TACAACGCAGAAGGGGTGTC-3′	55	Tashiro et al. (66)
	RP: 5′-CCTCAGCAACAAGTTCGACG-3′
Collagen III	FP: 5′-ATGGTGGCTTTCAGTTCAGC-3	55	Tashiro et al. (66)
	RP: 5′-TGTCTTGCTCCATTCACCAG-3′
Collagen V	FP: 5′-AGGGACCAACTGGGAAGAGT-3′	55	Li et al. (26)
	RP: 5′-AAAGTCAGAGGCAGCCAGAT-3′
Collagen VI	FP: 5′-AACGTGTGGCTAACCTGGAG-3′	53	Designed
	RP: 5′-TATTATCAAGCTGCTCCGCC-3′
Collagen VI	FP: 5′-TGCTCACCTGAGGCGCTTGC-3′	61	Designed
	RP: 5′-CCGCCTGCCTCCGTGAAGTG-3′
Collagen XI	FP: 5′-TCCGCCTGGAGCTTCCGGTT-3′	61	Designed
	RP: 5′-GACCCTGAGGTCCGACCGGG-3′
Collagen XXVII	FP: 5′-GTCAGATTGGAGCAGGGGTA-3′	55	Designed
	RP: 5′-ATGCTCTGGATGAGGTTGCT-3′
Cthrc1	FP: 5′-TTCAGGACCTCTTCCCATTG-3′	54	Designed
	RP: 5′-CACAGAGTCCTTCCACGGAG-3′
Mmp3	FP: 5′-CTCTCTCAAGATGATGTAGATGGTATTC-3′	53	Maeda et al. (33)
	RP: 5′-AGCTACACATTGGTAAGGTCTCAG-3′
Mmp11	FP: 5′-GGAGACTATTGGCGTTTCCA-3′	53	Designed
	RP: 5′-AGTAGGCATAGCCCTCAGCA-3′
Mmp12	FP: 5′-CTGGGCAACTGGACACCT-3′	60	Wasserman et al. (74)
	RP: 5′-CTACATCCGCACGCTTCA-3′
Mmp13	FP: 5′-GCATGAAAACTGTGGGGAGT-3′	58	Fang et al. (11)
	RP: 5′-AGCTGAAATCTTGCCTTGGA-3′
Mmp14	FP: 5′-GAGGGTCATGAGAAGCAGGC-3′	56	Strup-Perrot et al. (64)
	RP: 5′-TCAAAGGGTGTGCTGTCGC-3′
Mmp19	FP: 5′-AGGATACTGGCAATGGGATG-3′	53	Designed
	RP: 5′-TAGCTGCTGAGGGTTGGTCT-3′
Timp1	FP: 5′-GTTCCCTGGCATAATCTGAG-3′	52	Johnson et al. (18)
	RP: 5′-GGGATGAGGATCTGATCTGT-3′
Postn	FP: 5′-AGGAGCCGTGTTTGAGACCAT-3′	54	Hiroi et al.
	RP: 5′-CGGTGAAAGTGGTTTGCTGTTT-3′
Prss35	FP: 5′-TAGGAGAAGGAGGCAGGTGTATGG-3′	53	Miyakoshi et al. (37)
	RP: 5′-TAAGCACAGTTGGCAGCGTTTCCG-3′

AT, annealing temperature; FP, forward primer; RP, reverse primer.