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. 2011 Jun 8;31(23):8491–8501. doi: 10.1523/JNEUROSCI.5317-10.2011

Figure 1.

Figure 1.

Distinct expression patterns of NRG1 types in the human cerebral cortex. A, Diagram of NRG1 gene structure. Type-specific forward primers (indicated by arrows) for each type were located in unique exons, whereas reverse primers were located in either Ig or EGF domain. Forward primers were also designed for EGF domain. B, Agarose gel electrophoresis of RT-qPCR products. Total RNA of human cerebral cortex was used as template in RT-qPCR with specific primers. Products were resolved on 3% agarose gel and visualized by ethidium bromide staining. Bands were at the anticipated molecular weight. Due to potential difference in PCR efficiencies, band intensity did not faithfully indicate relative amount. C, Different levels of NRG1 types in the cerebral cortex of a 13-year-old subject. D, Composition of NRG1 types in the cerebral cortex of a 13-year-old subject. E, Different levels of NRG1 types in the cerebral cortex of a 60-year-old subject. The copy numbers per microgram total RNA of each type NRG1 were 1.67 ± 0.17 × 104 (type I), 8.31 ± 1.17 × 104 (type II), 2.93 ± 0.23 × 105 (type III), 1.31 ± 0.06 × 103 (type IV), 5.12 ± 0.73 × 103 (type V), and 227 ± 226 (type VI). F, Composition of NRG1 types in the cerebral cortex of a 60-year-old subject.