Figure 2.

Expression of all six types of NRG1 in rat brain. RT-qPCR was performed using the same strategy as described in Figure 1A. A, Agarose gel electrophoresis of RT-qPCR products. Total RNA of E18 rat brain was used as template in RT-qPCR with specific primers. Products were resolved on 3% agarose gel and visualized by ethidium bromide staining. Bands were at the anticipated molecular weight. Due to potential difference in PCR efficiencies, band intensity did not faithfully indicate relative amount. B, DNA sequence analysis of RT-qPCR products. RT-qPCR products were purified and subcloned into pGEM-T easy vector (Promega) for sequencing with T7 primer. Shown were partial DNA sequences of respective domains.