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Effect of 14 days of cold exposure (4°C) on amount of pan AMPK (A), P-AMPK (B), and P-AMPK-to-pan AMPK ratio (C) in WAT assessed by Western blot analysis in wild-type and α1-AMPK−/− mice. Values are means ± SE (n = 6–8/group). D: representative blots for pan AMPK and P-AMPK.