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. Author manuscript; available in PMC: 2011 Aug 11.
Published in final edited form as: J Infect Dis. 2007 Dec 15;196(12):1844–1851. doi: 10.1086/522968

Figure 1.

Figure 1

Migration to the spleen and stimulation of T cell proliferation by intraperitoneally injected purified eosinophils. In panel A, 5 × 106 carboxyfluorescein succinimide ester (CFSE)–labeled eosinophils were inoculated intraperitoneally into naive C57BL/6J mice. Spleens were recovered 36 h later, made into single-cell suspensions, and evaluated for the presence of CFSE-labeled eosinophils by use of a FACSCalibur flow cytometer. Representative data from 1 of 2 separate experiments are shown. In panel B, 5 × 105 control eosinophils (indicated as “EOS” in the figure), eosinophils pulsed with Strongyloides stercoralis antigen (SAg), or eosinophils pulsed with lipopolysaccharide (LPS) were intraperitoneally inoculated into naive C57BL/6J mice. On day 10, spleen cells were labeled with CFSE and cultured in the presence of anti-CD3 antibody (αCD3) to demonstrate T cell proliferation. Representative data from 1 of 3 separate experiments are shown.