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. Author manuscript; available in PMC: 2012 May 2.
Published in final edited form as: Chembiochem. 2011 Apr 5;12(7):1084–1096. doi: 10.1002/cbic.201000598

Figure 2. Characterization of D. melanogaster CSD dimerization by AUC and Far-UV CD.

Figure 2

Equilibrium AUC absorbance curves (bottom plot) were globally fit to a monomer-dimer model to obtain the converged dissociation constant Kd with the residual plot shown (top plot) for wild-type CSD (A), and its mutants W200A (C) and Δ204 (D). Data for the mutant I191E polypeptide converged with a single-species fit, exhibiting a molecular weight of 9.62 kD, as expected for a single monomer species; residuals are shown in the upper panel (B). E. A superposition of the far-UV CD spectra of wild-type and I191E CSD polypeptides indicates no difference in the secondary structure, even though the I191E CSD construct does not dimerize.