Fig. 1.

Photomicrographs of cell patching and identification of patched cells by immunocytochemistry. A: bright-field view of a brain slice containing the suprachiasmatic nucleus (SCN) with the recording pipette (white arrow) in position. Scale bar = 100 μm. V, 3rd ventricle; OC, optic chiasm. B: green fluorescent protein (GFP) cells seen during patch-clamping under fluorescent light with a GFP filter. The black arrow indicates the patched cell (scale bar = 30 μm). The inset shows the electrode (white arrow) patching a GFP-labeled (GFP+) cell (black arrow; scale bar = 20 μm). C: confocal images of 2 patched cells stained for biocytin (green) and GFP (red) and the overlay, confirming the peptidergic identity of the cell (white arrows indicate location of the filled cell). The cell in the 1st row is a GFP+ cell, and the cell in the 2nd row is a non-GFP cell (GFP−; z-axis = 2 μm; scale bar = 10 μm).