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. 2011 Aug 11;7(8):e1002177. doi: 10.1371/journal.ppat.1002177

Figure 6. Quantitative real-time PCR analysis of Hxl1-dependent regulation of putative UPR genes.

Figure 6

Strains were grown in YPD medium to early exponential phase, exposed to TM (5 µg/ml) for 1 hr (A–D) or to high temperature at 37°C for 2 hr (E), and harvested. The relative expression levels of putative UPR-regulated genes were analyzed by qRT-PCR with primers listed in Table S1 and normalized to that of ACT1. Strains were wild-type (H99), hxl1 mutant strain (YSB723), and ire1 mutant (YSB552). Error bars represent standard deviation (SD) of triplicated (A–D) or duplicated (E).