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The reaction system of RT-LAMP assay
| Reaction component | Reaction volume of H3-RT-LAMP assay |
|
|---|---|---|
| 10 × Thermopol Buffer | 2.5 μL | |
| MgSO4 | 5 mmol/L | |
| Betaine | 1 mmol/L | |
| F3 | 0.2 μmol/L | |
| B3 | 0.2 μmol/L | |
| primer | FIP | 1.6 μmol/L |
| BIP | 1.6 μmol/L | |
| LF | 0.8 μmol/L | |
| LB | 0.8 μmol/L | |
| dNTP | 1.4 mmol/L | |
| Calcein | 25 μmol/L | |
| MnCl2 | 0.5 mmol/L | |
| M-MuLV RTase | 200U | |
| Bst DNA polymerase | 8U | |
| Template RNA | 2 μL | |
| Nuclease-free water | Up to 25 μL | |
