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. 2011 Jul 18;11:197. doi: 10.1186/1471-2334-11-197

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Copyright ©2011 Shi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The optimal condition of RT-LAMP for the detection of EV71. (2A) The optimal temperature of RT-LAMP assay is monitored by agarose gel analysis. M: Marker, 100-bp DNA ladder (Sigma, USA); Lanes 1-6 are reaction temperatures at 60, 61, 62, 63, 64 and 65°C, respectively. (2B) The reaction time of RT-LAMP. Lanes 1-5 are reaction time of 30, 40, 50, 60 and 70 min, respectively. (2C) The concentration of magnesium. Lanes 1-5 are magnesium at the concentrations of 0, 1, 2, 2.8 and 3.6 mM, respectively. (2D) The concentration of betaine. Lanes 1-4 are the betaine concentrations of 0, 0.5, 1.0 and 1.5 M, respectively.