TABLE 1:
Relationship between stoichiometry of Hsp/c70 to CBag and stability of full-length CFTR.
| Hsc70 (μM) | Hsp70 (μM) | Total Hsp/c70 (μM) | Exogenous CBag or Bag-1 (μM) | Total Hsp/c70 : Exogenous CBag or Bag-1 | T1/2 of full-length CFTR (min) | |
|---|---|---|---|---|---|---|
| RRL | 2 | n.d. | 2 | – | – | 25 |
| RRL + 2 μM CBag | 2 | n.d. | 2 | 2 | 1 : 1 | 34 |
| RRL + 5 μM CBag | 2 | n.d. | 2 | 5 | 1 : 2.5 | 55 |
| RRL + 10 μM CBag | 2 | n.d. | 2 | 10 | 1 : 5 | 94 |
| RRL + 20 μM CBag | 2 | n.d. | 2 | 20 | 1 : 10 | 116 |
| HEK293 | 12 | 24 | 36 | - | – | 50 |
| HEK293 + CBag | 12 | 24 | 36 | 1.6 | 1 : 0.04 | 53 |
| HEK293 + Bag-1 | 12 | 24 | 36 | 4.8 | 1 : 0.13 | n.e. |
| COSm6 | 12 | n.d. | 12 | - | n.e. | |
| COSm6 + CBag | 12 | n.d. | 12 | 1.6 | 1 : 0.13 | n.e. |
T1/2 values in RRL are from stability of full-length, wild-type CFTR in in vitro degradation assays (Supplemental Figure 3), whereas those in HEK293 cells are from stability of band B form of ΔF508 CFTR in pulse-chase assays (Supplemental Figure 8). n.d., not detected; n.e., not examined.