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. 2011 Aug 15;22(16):2848–2861. doi: 10.1091/mbc.E11-04-0378

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© 2011 Tsuchiya et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 4: — In the mad2Δ cells that undergo one meiotic division, protection of the meiotic cohesin Rec8 is lost prematurely. (A) Time lapse images of meiosis in wild-type and 1 division mad2Δ/mad2Δ cells. Cells are expressing Tub1-mCherry and Rec8-GFP. A green arrow marks the time of the first Rec8 cleavage. The time from first cleavage to anaphase I was calculated (± SD). (B) Time lapse images of meiosis in wild-type and 1 division mad2Δ/mad2Δ cells. Cells are expressing Tub1-mCherry and Sgo1-GFP. A green arrow marks the time at which Sgo1 is lost in the 1 division mad2Δ cells. One hundred wild-type cells and 50 mad2Δ cells that formed dyads were analyzed.