TABLE 3:
Strains used in this study.
| Strain name | Genotype |
|---|---|
| LY56 | MATa/α, LacO:TRP1/LacO:TRP1, PTUB1-GFP-TUB1-URA3/PCYC1-GFP-LacI2-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15 |
| LY7 | MATa/α, mad2::LEU2/mad2::LEU2, LacO:TRP1/LacO:TRP1, PCYC1-GFP-LacI2-URA3/ura3-1, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15 |
| LY9 | MATa/α, mad2::LEU2/mad2::LEU2, LacO:TRP1/trp1-1, PCYC1-GFP-LacI2-URA3/ura3-1, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15 |
| LY806 | MATa/α, mad2::LEU2/mad2::kanMX4, PTUB1-GFP-TUB1-URA3/PCYC1-GFP-LacI2-URA3, ZIP1-GFP-700/ZIP1, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY838 | MATa/α, PTUB1-GFP-TUB1-URA3/ura3-1, ZIP1-GFP-700/ZIP1, LacO:TRP1/trp1-1, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, |
| LY849 | MATa/α, mad2:: kanMX4/mad2::kanMX4, PCYC1-GFP-LacI2-URA3/ura3-1, LacO:LEU2/leu2-3112, ade2-1/ade2-1, can1-100/can1-100, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY850 | MATa/α, mad2:: kanMX4/mad2::kanMX4, PCYC1-GFP-LacI2-URA3/ura3-1, LacO:TRP1(at chromIV genomic fragment 550857)/+, leu2-3112/leu2-3112, ade2-1/ade2-1, can1-100/can1-100, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY851 | MATa/α, mad2:: kanMX4/mad2::kanMX4, PCYC1-GFP-LacI2-URA3/ura3-1, LacO:TRP1(at chromIV genomic fragment 793927)/+, leu2-3112/leu2-3112, ade2-1/ade2-1, can1-100/can1-100, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY578 | MATa/α, mad2::LEU2/mad2::LEU2, LRS4-GFP-HIS3/LRS4-GFP-HIS3, LacO:TRP1/LacO:TRP1, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15 |
| LY579 | MATa/α, LRS4-GFP-HIS3/LRS4-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY500 | MATa/α, mad2::kanMX4/mad2::kanMX4, REC8-GFP-kanMX4/REC8-GFP-kanMX4, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY527 | MATa/α, REC8-GFP-kanMX4/REC8-GFP-kanMX4, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY685 | MATa/α, mad2::kanMX4/mad2::kanMX4, SGO1-GFP-HIS3/SGO1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY684 | MATa/α, SGO1-GFP-HIS3/SGO1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY389 | MATa/α, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY358 | MATa/α, mad2::kanMX4/mad2::kanMX4, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY768 | MATa/α, mad2::LEU2/mad2::LEU2, swm1::kanMX4/swm1::kanMX4, LacO:TRP1/LacO:TRP1, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY774 | MATa/α, swm1::kanMX4/swm1::kanMX4, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY528 | MATa/α, CDC14-GFP-HIS3/CDC14-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, PHIS3-mCherry-TUB1-ADE2/PHIS3-mCherry-TUB1-ADE2, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY411 | MATa/α, mad2::LEU2/mad2::LEU2, CDC14-GFP-HIS3/CDC14-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY698 | MATa/α, mad2::LEU2/mad2::LEU2, ama1::kanMX4/ama1::kanMX4, CDC14-GFP-HIS3/CDC14-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY274 | MATa/α, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, PCLB2-3HA-CDC20-kanMX6/PCLB2-3HA-CDC20-kanMX6, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY720 | MATa/α, mad2::LEU2/mad2::LEU2, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3 (at mad2)/PHIS3-mCherry-TUB1-URA3 (at mad2), PCLB2-3HA-CDC20-kanMX6/PCLB2-3HA-CDC20-kanMX6, PCYC1-GFP-LacI2-URA3/, PCYC1-GFP-LacI2-URA3, LacO:TRP1/trp1-1 ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15 |
| LY752 | MATa/α, mad3::kanMX4/mad3::kanMX4, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, PHIS3-mCherry-TUB1-ADE2/PHIS3-mCherry-TUB1-ADE2, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY689 | MATa/α, cdc20-127/cdc20-127, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY657 | MATa/α, ama1::kanMX4/ama1::kanMX4, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY643 | MATa/α, mad2::LEU2/mad2::LEU2, ama1::kanMX4/ama1::kanMX4, LacO:TRP1/LacO:TRP1, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY829 | MATa/α, mad2::kanMX/mad2::kanMX, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, PAma1-Ama1-m8-LEU2/PAma1-Ama1-m8-LEU2 ade2-1/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
| LY802 | MATa/α, PDS1-GFP-HIS3/PDS1-GFP-HIS3, PHIS3-mCherry-TUB1-URA3/PHIS3-mCherry-TUB1-URA3, ama1::kanMX/ama1::kanMX PAma1-Ama1-m8-LEU2/PAma1-Ama1-m8-LEU2, PHIS3-mCherry-TUB1-ADE2/ade2-1, can1-100/can1-100, leu2-3112/leu2-3112, his3-11,15/his3-11,15, trp1-1/trp1-1 |
All strains are derivatives of W303 (ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100).