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. 2011 Aug 15;22(16):2886–2899. doi: 10.1091/mbc.E10-10-0862

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© 2011 Bernadskaya et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2: — Arp2/3 maintains apical organization during postembryonic growth. (A) Adult intestines are shown by differential interference contrast (DIC) (top) and fluorescence optics (bottom). Split arrows indicate intestinal lumen width. Graph shows the lumen width of young adults in wild-type animals and in animals depleted of the WAVE-complex component GEX-3, ARP-2, or apical junction components. A strain that specifically marks the intestinal cells with GFP (vha-6p::GFP) facilitated measurement of the lumen width. (B) Postembryonic apical F-actin levels were detected with phalloidin (green) in dissected and fixed intestines. DAPI (red) indicates intestinal nuclei. Graphs: n > 15 adults for each genotype. Error bars show SEM. Asterisks mark statistical significance, p < 0.05. Values on the y-axis indicate fluorescence intensity in arbitrary units here and throughout the figures.