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. 2011 Aug 15;22(16):2924–2936. doi: 10.1091/mbc.E11-04-0294

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© 2011 Castillon et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2: — Emp24p is not required for GPI-anchored cargo sorting and concentration into ERES. (A) Fluorescence micrographs of live sec31-1 and sec31-1 erv14Δ cells expressing Hxt1-CFP at 37ºC. Raw images. (B) Quantification of several micrographs described in A. The graph plots the average percentage of the cells, for which Hxt1-CFP is found in dot-like structures. n, number of cells plotted; 74 ≤ n ≤ 89. (C) Fluorescence micrographs of live sec31-1, sec31-1 bst1Δ, and sec31-1 emp24Δ cells expressing Cwp2-Venus at 37ºC. Raw images. (D) Quantification of several micrographs described in C. The graph plots the average percentage of the cells, for which Cwp2-Venus is found in dot-like structures. n, number of cells plotted; 74 ≤ n ≤ 89. (E) Fluorescence micrographs of live sec31-1 and sec31-1 emp24Δ cells expressing Cwp2-Venus (green) and Sec13-mCh (red) at 37°C. White arrowheads, colocalizing dots. Deconvoluted images by 10 iterations. (F) Quantification of several micrographs described in E. The graph displays the means of the percentage of colocalization per cell of Cwp2-Venus dots with Sec13-mCh dots in sec31-1 (black bars, n = 36) and in sec31-1 emp24Δ (white bars, n = 55). Scale bar, A, C, E, 5 μm.