FIGURE 3:

Degradation of Erg3p requires Htm1p and Yos9p but not Der1p. (A) Exponentially growing cells of the indicated yeast strains were pulse-labeled with ³⁵S for 15 min, and equal aliquots were removed at the specified time points. Erg3p-myc was immunoprecipitated from cell lysates, and samples were subsequently separated by SDS–PAGE. A PhosphorImager scan of a typical gel is shown. (B) Erg3p-myc degradation was analyzed in the indicated yeast strains. Five independent experiments were quantified using a PhosphorImager, and the results were averaged. The errors bars indicate the SE of the experiments. (C) Stability of CPY* was analyzed in the indicated yeast strains. At least four independent experiments were quantified for each strain using a PhosphorImager, and the results were averaged. The errors bars indicate the SE of the experiments.