Table 1.

PCR primers used for CpG methylation profiling of rat Isyna1.

Region	Primer name	CpG†	Sequence‡
Region 1	rM-Isyn 313	1–9	GATGAGTTTAGTTGGGTTGG
	rM-Isyn 553		ACCTCCCCCTAAACAA
Region 2§	rM-Isyn 611	11–31	ATTAGGAGTAGGTAGGATAAT
	rM-Isyn 930		CTTACCRACTAAAAATTAAATCAAAC
	rM-Isyn 905	31–43	GTTTGATTTAATTTTTAGTY
	rM-Isyn 1046		RAACTATCCACCAAAATCTC
	rM-Isyn 1027	44–70	GAGATTTTGGTGGATAGTTY
	rM-Isyn 1203		AAAAATAAATAAAAAATAAATCR
Region 3	rM-Isyn 1415	89–106	GTGGTTGTTAGGAGGA
	rM-Isyn 1770		AACACCAAACCCATCC

^†Refers to the position of CpG residues in the 1.35-kb fragment (see Figure 1).

^‡Primers used on bisulfite-modified DNA.

^§CpG profile for region 2 was derived from three overlapping amplicons.

R: A/G; Y: C/T.