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(A) Schematic of wildtype and resulting LMP1Δ viruses. EBV coordinates according to B958 numbering. Primers for PCR screening indicated. (B) PCR across the LMP1 loci of the indicated BAC. (C) BAMH1 digest indicates the wt and LMP1Δ BAC genomes have no gross deletion. A unique 8.2 kB fragment (*) corresponds to the expected size of the BAM N fragment harboring the LMP1 deletion.
