Skip to main content
. 2011 Aug 11;6(8):e23076. doi: 10.1371/journal.pone.0023076

Figure 1. Construction of stable human FPR3-inducible HEK293S cell lines using the T-REx system (Life Technologies).

Figure 1

Clones were tested for induction after 48 h in plain media (−) or media supplemented with 1 µg/ml tetracycline (+) or tetracycline plus 2.5 mM sodium butyrate enhancer (++). Arrows indicate the positions of the 38- and 42-kDa monomer forms. Levels of human FPR3 were probed via Western blot analysis against the rho1D4 tag at the C-terminus of the protein sequence. Clones 1, 14 and 15 show highest levels of induction while maintaining undetectable background levels in the absence of tetracycline. Clone 15 that only expressed the 42-kDa FPR3 monomer was selected for subsequent experiments.