Figure 2. Detergent screening for optimal solubilization of human FPR3 expressed in HEK293S cells.

Expression of the FPR3 was induced with tetracycline (1 µg/ml) and sodium butyrate (2.5 mM) for 48 h and receptors were solubilized in PBS containing detergent(s) for 1 h at 4°C. In total, 96 detergents/detergent mixtures were screened in this study. Each integer from 1 to 96 along x-axis represents a detergent/detergent mixture (see Table S1 for more information about each detergent/detergent mixture). The ability of screened detergents/detergent mixtures to solubilize the FPR3 has been quantified by spot densitometry and normalized to the range from 0.0 to 1.0 for ease of comparison.