Figure 3. A) Gel filtration on immunoaffinity-purified human FPR3 from ∼6 g cells (wet weight).

Absorbance was recorded at 280 nm (black line), 254 nm (gray line), and 215 nm (dashed line). Peak 1–4 (indicated by numbers) were pooled and concentrated. Peak 5 contained the rho1D4 elution peptide TETSQVAPA from the immunoaffinity purification. B) Silver staining of the peak fractions. Peak numbers refer to those designated in (A).