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. 2011 Aug 11;6(8):e23376. doi: 10.1371/journal.pone.0023376

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Exchange constructs harbouring the promoter-luciferase cassettes in either sense or antisense orientation were targeted to the ROSA26 locus via PhiC31 integrase mediated cassette exchange. (B) A Fluc coding sequence was targeted to the ROSA26 locus via PhiC31 integrase mediated cassette exchange. Flp recombinase mediated deletion of the selection cassette, brought the luciferase cassette into the context of the ROSA26 forward transcript via the 5′ Splice Acceptor (SA).