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. 2011 Aug 11;6(8):e23376. doi: 10.1371/journal.pone.0023376

Figure 2. Relative promoter activities of Fluc reporter constructs stably integrated at ROSA26.

Figure 2

Fluc expression cassettes under the control of different promoters were inserted into the ROSA26 locus in both sense and antisense orientation by PhiC31 integrase mediated cassette exchange. Fluc activity from the exogenous promoters is expressed relative to that of luciferase under the control of the endogenous ROSA26 promoter (ROSA26>Fluc). “Fluc Only” represents ES cells harbouring the promoterless construct, ROSA26>Neo>Fluc represents ES cells harbouring inactive ROSA26 driven Fluc, prior to Flp deletion, and IDG26.10-3 ES cells are the “wild-type” ES cells used prior to the transgene insertion. Using multilevel modelling, the difference in activities between promoter-luciferase cassettes positioned in the sense and antisense orientations were found to be highly significant for EF1α (***p = 0.0007) and significant for UbC and MC1(*). Fluc assays were normalized to total protein concentrations and error bars represent the standard error of the mean from at least 3 independent experiments.