Table 1. Kinetic parameters of CD39L2.
Substrate | Km (mM) | kcat(s-1) | kcat/Km (s−1 mM−1) | Vmax(nmol min−1 µg−1) |
ADP | 1.05 | 38.5 | 36.6 | 49.1 |
ATP | 1.14 | 0.617 | 0.727 | 0.928 |
The kinetic data were measured in the buffer of 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, and 10 mM CaCl2 at pH 7.5 and room temperature. For accuracy, the substrate consumption rate in all reactions was kept below 12%.