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. 2011 Aug 11;7(8):e1002229. doi: 10.1371/journal.pgen.1002229

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Kung et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The protein levels of GS in MCF7 under different concentrations of glutamine. (B, C) The changes of the levels of glutamine in medium (B) and intracellular glutamine concentrations (C) in MCF7 and MDAMB231 cells deprived of glutamine for 12 and 24 h. (D) A diagram illustrating the co-culture systems in E, F. (E, F) The cell viability (E) and the glutamine levels in medium (F) when used to propagate MDAMB231 cells co-cultured with MDAMB231 or MCF7. (G) A diagram illustrating the condition medium model for H, I. (H, I) The cell viability (H) and the glutamine in medium (I) in MDAMB231 cells cultured with fresh medium, MDAMB231 or MCF7 condition medium. (J) A diagram illustrating the model of K. (K) The intracellular glutamine in vector transfected MDAMB231 cells. (L) A diagram illustrating the model of co-culture system for M-O. (M-O) The cell viability (M), the glutamine in medium (N), and intracellular glutamine concentrations (O) in MDAMB231 co-cultured with transfected MDAMB231 cells.