CHI3L1 membrane association and peri-nucreic accumulation is C-terminal chitin-binding domain dependent. A: Schema of CHI3L1 and its deletion mutants in the Ce-terminal chitin-binding motif/domain (CBD). B: COS7 cells were transiently transfected with pCDNA4 HisMax CHI3L1 WT vector, stained with FITC-anti-Xpress antibody (left), Texas Red-calnexin antibody (center), and both as merged image (right), which were analyzed by confocal microscope. Bars, 20 µm. C: COS7 and SW480 cells were transfected with pcDNA HisMax Lacz (control), CHI3L1 WT or CHI3L1 deletion-mutants (ΔCBD, Δ325, and Δ339), were stained with FITC anti- Xpress and Texas Red-anti-calnexin antibodies, and were analyzed by confocal microscope. Magnifications: 240X, objective for COS7 cells, 180×, objective for SW480 cells.