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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Clin Immunol. 2011 Apr 20;140(3):268–275. doi: 10.1016/j.clim.2011.04.007

Figure 4.

Figure 4

CHI3L1 Chitin-binding motif/domain enhances the specific phosphorylation of Akt Thr 308 in SW480 cells. (A) Phospho- and total-Erk (MAPKp42/p44), MAPKp38, JNK/SAPK, AKT Thr 308, and AKT Ser 473 were analyzed by western blot analysis in SW480 cells which were transiently transfected with/without pcDNA4 LacZ vector (control), CHI3L1 (WT) or ΔCBD-expression vector. Phospho- and total-Akt Thr 308 were detected by western blot after transfected with pcDNA4 Lacz (control), CHI3L1 (WT) or CHI3L1 deletion-mutants (ΔCBD, Δ325, or Δ339) (B) or transfected with pcDNA4 Lacz (cont)-, ΔCBD- or CHI3L1 (WT) or expression vector pre-treating with anti-CHI3L1 Ab (250 µg/ml; shown as WT/Ab) or normal rabbit IgG (250 µg/ml; shown as WT/IgG) (C) in SW480 cells. Proteins were isolated 36–48 hours after the transfection with a lysis buffer as detailed in Materials & Methods.