Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Thromb Haemost. 2011 Aug;9(8):1562–1571. doi: 10.1111/j.1538-7836.2011.04394.x

Fig. 5.

Fig. 5

Effect of temperature on platelet signaling. WPs were preincubated at 37°, 40° or 42°C for 10 minutes. (A) WPs were stimulated with the indicated concentration of thrombin for 5 minutes and lysates were immunoblotted with anti-pp38, pERK1/2, pHSP27 or pAKT antibody. (B) WPs were stimulated with thrombin for 2 minutes and lysates were immunoblotted with anti-pSyk and pSrc antibody. Each membrane was reprobed with anti-actin antibody to calculate the relative IOD using Gel-Pro software (n=3–5). *P < 0.05 vs. thrombin at 37°C.