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. 2011 Aug 10;19(8):1127–1137. doi: 10.1016/j.str.2011.06.003

Figure 1.

Figure 1

Kinase Activity of p110δ in the Presence of p85α Constructs and PDGFR pY

(A) p110δ/p85α constructs tested for lipid kinase activity.

(B) Kinase activity of ΔABD-p110δ compared with p110δ /iSH2 p85α. Assays measured 32P-PIP3 production in the presence of 0.5 nM enzyme, 100 μM ATP, and 5% PIP2 lipid vesicles at a concentration of 1 mg/ml. The left panel illustrates the autoradiogram of the filter (duplicate spots) and the right shows the quantitation of the spots. Kinase assays were performed in duplicate and repeated twice. The error bars show the standard deviation (SD).

(C) In vitro kinase assay results for various p110δ and p85α constructs are shown. Assays measured 32P-PIP3 production in the presence of 5 nM enzyme, 100 μM ATP, and 5% PIP2 vesicles at a concentration of 1 mg/ml, +/− 10 μM PDGFR pY. Kinase assays were performed in duplicate and repeated twice.