FIG. 2.
Requirement of sodium channel activation and intracellular calcium for deltamethrin-induced apoptosis. Representative traces of ([Na+]i) (A) and Ca2+ (B) influx in SK-N-AS cells. Pretreatment with the Na+ channel antagonist TTX (1μM) prevented deltamethrin-induced DNA fragmentation (C). Chelation of intracellular calcium by BAPTA-AM prevented deltamethrin-induced DNA fragmentation (D and E). Data represent mean ± SEM of three to five individual experiments, each performed in triplicate and expressed as percentage of control. * indicates significantly different from control and # indicate significant differences between deltamethrin-treated cells and deltamethrin plus TTX or BAPTA-AM-treated cells (p < 0.05).