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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Future Med Chem. 2011 May;3(7):809–819. doi: 10.4155/fmc.11.48

Figure 1. Methods for introducing mutations into progenitor genes target the whole gene or particular sites are targeted rationally through structural or other functional data.

Figure 1

CASTing: Combinatorial active-site saturation testing; epPCR: Error prone polymerase chain reaction; ITCHY: incremental truncation of hybrid enzymes; SHIPREC: Sequence homology-independent recombination; StEP: Staggered extension process.