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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Future Med Chem. 2011 May;3(7):809–819. doi: 10.4155/fmc.11.48

Figure 2. Representative methods for identification of improved enzyme variants are classified by throughput capacity.

Figure 2

Examples of methodologies capable of selecting or screening ultra-large libraries include fluorescence-activated cell sorting, enrichment and phage display. High-throughput methods include selection or screening on agar plates. Many biochemical-assay methods can be modified to 96-well format, but require colonies to be picked into individual wells prior to screening. Some plate-based colorimetric screens require secondary analysis in this format.