Figure 1. Wnt5a and Wnt3a have opposite effects on neurite development.

Neurons are identified by staining with Tuj1 (red) and MAP2 (green) antibodies. DAPI counterstain is used to show the density of other cells in the culture. (A) Neurons treated with vehicle for 3 days as a control. (B) Cultured neurons treated with soluble Wnt5a. (C) Neurons treated with Wnt3a. (D) Neurons treated with non-specific Wnt inhibitor sFRP-1. (E) Neurons treated with canonical inhibitor DKK-1. (F) Quantification of TDBTN for each condition. All P values <0.001. Scale bar = 50 μm.