Figure 4. Wnt3a does not directly up-regulate the canonical BMP signalling pathway.

Rat cells were treated with Wnt3a or BMP4, cells were harvested for protein at 30 min and 3 h post treatment, and Western blots were performed. (A) At both time points, BMP4 bands are visible in the positive control conditions, as compared with untreated and Wnt3a-treated conditions. (B) At both time points, phosphorylated-Smad bands are visible in BMP4-treated conditions but not in untreated or Wnt3a-treated conditions. GAPDH was used as a loading control for both analyses.