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. 2011 Jun 16;3(3):e00061. doi: 10.1042/AN20110004

Figure 5. Collective treatment with BMP4 and Wnt3a does not inhibit precursor differentiation greater than individual treatment with BMP4 or Wnt3a.

Figure 5

Rat OPCs were cultured and then placed in DM with 50 ng/ml BMP4, 10 ng/ml BMP4, 50 ng/ml Wnt3a or 50 ng/ml Wnt3a and 10 ng/ml BMP4. After 3 days, 50 ng/ml BMP4 decreased GalC+ cells by 92% relative to control conditions (P<0.05), 10 ng/ml BMP4 decreased OPC differentiation by 35% (P<0.05), 50 ng/ml Wnt3a decreased OPC differentiation by 38% (P<0.05) and 50 ng/ml Wnt3a combined with 10 ng/ml BMP4 decreased OPC differentiation by 44% (P<0.05). The difference between the combined addition of BMP4 and Wnt3a was not significantly different from the application of either treatment factor individually (n = 3).