FIGURE 1.
The mRNA levels of Bmal1, VGCCα1C, and VGCCα1D are under circadian control in chick hearts. (A) The circadian rhythm of Bmal1 mRNA reached high values during the subjective night and low ones during the subjective day. *: Bmal1 levels were significantly higher at CT 16 and CT 20 compared to CT 0, 4, 8, and 24, *p < .05; n = 9/timepoint. (B) *: The mRNA levels of VGCCα1C were significantly higher at CT 12, 16, and 20 than CT 8, *p < .05; #: VGCCα1C mRNA levels were significantly higher at CT 12 and CT 20 than CT 4, #p < .05; n = 7/timepoint. (C) The mRNA level of VGCCα1D peaked at CT 12 and was significantly higher than the levels at CT 0, 4, 8, 9, 13, 14, 16, and 20, *p < 0.05; n = 7/timepoint. Comparisons were made using ANOVA with Tukey’s post hoc test across different circadian timepoints.