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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Chronobiol Int. 2010 Oct;27(9-10):1673–1696. doi: 10.3109/07420528.2010.514631

FIGURE 4.

FIGURE 4

Activities of Erk, p38, pAktthr308, and GSK3β are under circadian regulation. Immunoblot analysis showing phosphorylation of Erk (pErk), p38 (pp38), Aktthr308 (pAktthr308), Aktser473 (pAktser473), and GSK-3β (pGSK) in whole hearts harvested at different circadian times on the second day of DD. (A) The protein level of pErk showed a circadian rhythm with its peak at CT 9 that differed significantly from CT 1, 5, 13, and 17 (n = 8/timepoint). (B) The protein level of phosphorylated p38 (p38) showed a circadian rhythm with its peak at CT 9 that differed significantly from CT 17 (n = 7/timepoint). (C) The protein level of pAktthr308 displayed an ultradian rhythm with peaks at CT 4 and CT 12–16 (n = 4/timepoint). *: Significant differences between CT 4 and CT 8, CT 4, and CT 20; #: CT 12 is significantly different from CT 8 and CT 20; &: CT 16 is significantly different from CT 20. (D) The protein level of pAktser473 did not display any circadian rhythm (n = 4/timepoint). (E) The protein level of pGSK showed a circadian rhythm with its peak at CT 20 that was significantly different from CT 0 and CT 12 (n = 6/timepoint). All comparisons were made using ANOVA with Tukey’s post hoc test across different circadian timepoints, p < .05.