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Imaging iPSCs with GFP. A. A lentiviral vector which includes the four defined transcription factors and GFP, separated by an internal ribosome entry site (IRES) sequence. B. Tracking transgene expression with GFP during reprogramming. C. Significant contribution of iPSCs in a chimeric embryo, where GFP expression from donor iPSCs is evident. D. Sections of a chimeric embryo showed contribution of iPSCs to the brain and liver. Adapted from [28].
