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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Lett. 2011 Jun 24;310(1):15–24. doi: 10.1016/j.canlet.2011.05.024

Figure 3.

Figure 3

EMD534085-induced cell death in HL60 during mitotic arrest occurs by the intrinsic pathway. A. Caspase-8, −9, −3, −7 are activated; Parp1 is cleaved; Mcl1 and XIAP are degraded in EMD534085-treated HL60 cells. Cells were synchronized using single thymidine block, and then were released to either vehicle alone or 500nM EMD534085 supplemented growth medium. Cells were harvested 4, 6, 8, 10, 12, 14, and 16 hrs after thymidine release for immunoblotting. B. Cytochrome c translocates from mitochondrial inter-membrane space to the cytoplasm during mitotic arrest. cytochrome c – red; microtubule – green; DNA – blue. C. Bax is activated in HL60 during EMD534085 treatment. D. Bcl-2 overexpression protects HL60 cells from EMD534085 treatment. (*: p-value < 0.05)