Table 1. Substrate Spectrum of Pisa1 Compared to Results with Whole Cells of Pseudomonas sp. DSM 6611.
|
Ps. sp. DSM 6611a |
Pisa1 |
|||||
|---|---|---|---|---|---|---|
| substrate | R1 | R2 | c [%] | Eb | c [%] | Eb |
| rac-2a | CH3 | n-C6H13 | 21 | >200 | 50 | >200 |
| rac-3a | CH3 | n-C5H11 | 17 | >200 | 50 | >200 |
| rac-4a | CH3 | n-C7H15 | 7 | >200 | 50 | >200 |
| rac-5a | C2H5 | n-C5H11 | 18 | >200 | 50 | >200 |
| rac-6a | n-C3H7 | n-C4H9 | 20 | 6 | 57 | 10 |
| rac-7a | CH3 | (CH2)2CH=CMe2 | 9 | >200 | 50 | >200 |
| rac-8a | CH3 | CH2Ph | <1 | n.d. | 30 | >200 |
| rac-9a | CH3 | (CH2)2Ph | 15 | >200 | 50 | >200 |
| rac-10a | CH3 | c-C6H11 | 5 | >200 | 10 | >200 |
| rac-11a | HC≡C | n-C4H9 | n.i. | – | 50 | >200 |
| rac-12a | HC≡C | n-C5H11 | n.i. | – | 50 | >200 |
a
Whole resting cells of Pseudomonas sp. DSM 6611, data from ref (13).
b
Enantioselectivity expressed as Enantiomeric Ratio (E-value); n.d. = not determined due to exceedingly low conversion; n.i. = not investigated.