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. Author manuscript; available in PMC: 2011 Aug 12.
Published in final edited form as: Mol Cancer Res. 2009 Jun 30;7(7):1021–1032. doi: 10.1158/1541-7786.MCR-08-0582

Fig 6. EphA2 impairment potently inhibits cell migration to an extent similar to GA.

Fig 6

(Upper panel) U251 cells were pretreated with EphA2 antibody (10 ug/ml) or GA (1 uM) 8 h prior to plating in Boyden chambers (ligand was added at time of plating), and cells were transfected with siRNAs 48 h prior to plating. Following a 16 h incubation period, migrating cells were counted as described and normalized to the number of migrating cells from DMSO treated cells. Values corresponding to the normalized mean were converted to percent inhibition and subjected to statistical analysis. Representative siRNA-mediated knockdown of EphA2 is shown. PC3 cells (lower panel) were treated as above and the percent migration was similarly calculated.