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CDK1 and Plk1 are necessary for Nek9 activation during mitosis. HeLa cells were arrested in mitosis with nocodazole. Mitotic cells were collected, washed and released in media containing MG132 (20 μM) plus DMSO, Purvalanol A (20 μM) or Bi2536 (100 nM) for 2 h, and cell extracts were analysed by western blot (WB) using the indicated antibodies. Untreated cells are shown in the first lane as a control. Asterisks mark protein bands with altered mobility due to phosphorylation.
