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. 2011 Jun 29;10:51. doi: 10.1186/1475-2859-10-51

Figure 4.

Figure 4

Far-UV CD spectra of U24 obtained from the different cell strains used in this study. U24 protein that had been isolated from various E. coli cell strains was reconstituted in 10 mM Tris·HCl, 10 mM NaCl, 10 mM SDS, pH 7.5 and far-UV CD was run in the presence and absence of tris(2-carboxyethyl)phosphine (TCEP) reducing agent. Concentration of protein in the samples were determined by BCA assay to be [U24]C41(DE3) = 0.106 ± 0.004 mg/ml, [U24]Origami = 0.093 ± 0.006 mg/ml, and [U24]SHuffle = 0.118 ± 0.004 mg/ml, where the standard deviation is based on four independent assay measurements.