Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Examination of cysteine-free mutant U24 expression. The C21SC37S mutant U24 constructs were expressed: pMAL-p2x-U24 in C41 (DE3) and pMAL-c2x-U24 in Origami 2 cells, at 18°C and 37°C. Removal of disulfide bond potential appeared to have no effect on in vivo stability of expressed MBP-6 × His-U24, which exhibited the same expression characteristics as wild-type; a similar loss in mass of the degraded fusion protein is observed at the higher temperature.
