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. 2011 Aug 12;6(8):e23042. doi: 10.1371/journal.pone.0023042

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Souza et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chromosomal bands were separated by Pulsed Field Gel Electrophoresis (PFGE) and stained with ethidium bromide. The chromosomal bands of clone CL Brener were numbered using Roman numerals (I – XX) and Dm28c with Arabic numerals as described previously [17], [18]. The bands of others isolates were numbered using Arabic numerals starting from the smallest band, as the reference determined for clone CL Brener [18]. The following isolates were used: Y strain and clone Esmeraldo-cl3 from lineage TcII; clone SO3-cl5 from TcV; CL Brener from TcVI; clone Dm28c, G strain and Tc1161 (José-IMT) isolate from TcI. Numbers of chromosomal bands and their sizes are indicated at left and at right of each strip, respectively.