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. 2011 Aug 12;6(8):e23042. doi: 10.1371/journal.pone.0023042

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Souza et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Linkage groups from TcChr37 and TcChr4 (Panel A) and TcChr39 (Panel B) were mapped on chromosomal bands. Specific markers from in silico assembled T. cruzi chromosomes (TcChr) were hybridized with chromosomal bands separated by PFGE. The positions of markers used as probes are indicated in the diagrammatic representation of in silico assembled chromosomes (TcChr37 and 4, and TcChr39).The isolates are: Y and clone Esmeraldo-cl3 (E) from lineage TcII; clone SO3-cl5 (S) from TcV; clone CL Brener (C) from TcVI; clone Dm28c (D), G strain and Tc1161 (José-IMT) isolate (T) from TcI. Markers from TcChr37: XM_801570, THTc, TEUF0001, TEUF0180, TcProcyclic, DEAD-H and delta-6. The marker 40S is located in TcChr4. Markers from TcChr39: XM_811753, H49, PI3,5K, XM_811099, JL8, katanin, iron-sulfur, syntaxin and ankyrin. The gene identification and GenBank accession number of each marker are indicated in Table S4.