Table 1. Total DNA content and genome size of various T. cruzi isolates.

Isolate	Group	Total DNA content* (ρg)			Genome size** (Mb)	Nuclear genome*** (Mb)
G	I	0.122270	±	0.026692	112.17	90
Dm28c	I	0.121429	±	0.031253	111.40	89
Tc1161	I	0.157183	±	0.031282	144.20	115
Y	II	0.171660	±	0.044942	157.49	126
Esmeraldo	II	0.155235	±	0.029427	142.42	114
SO3-cl5	V	0.183778	±	0.024398	168.60	135
CL	VI	0.191118	±	0.038978	175.34	140
CL14	VI	0.152632	±	0.015553	140.03	112
CL Brener	VI	0.165813	±	0.031826	152.12	122

*Absolute DNA mass (ρg) by means of fluorescent nucleic acid stain assay per epimastigote cell. Values are the means ± SD of three to seven independent assays performed in triplicate.

**Estimate of genome size including nuclear and kDNA considering 1 base pair as 1.09×10⁻⁹ ρg.

^***Nuclear genome size was estimated assuming that kDNA accounts for 20% of total DNA [45].