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. 2011 Aug 12;6(8):e23329. doi: 10.1371/journal.pone.0023329

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Kikkawa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Binding of Lu-Fc to laminin α5 in adult mouse kidney sections. Sections were incubated with Lu-Fc (upper panel) or Fc (lower panel) at room temperature for 1 hour. Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. Merged pictures showed that laminin α5 and Lu-Fc co-localized at basement membranes. Arrows indicate glomeruli, laminin α5-rich structures. Bar, 100 µm. (B) Binding of Lu-Fc to laminin α5 in adult mouse skeletal muscle sections. Endogenous laminin α2 (upper panel) and α5 (lower panel) were detected with antisera. Bound Lu-Fc was detected with an antibody against human IgG as described above. Lu-Fc only bound to the basement membranes containing α5, primarily neuromuscular junctions. Arrowheads indicate neuromuscular junctions. Bar, 50 µm. (C) Inhibitory effects of anti-Lu/B-CAM antibodies on Lu-Fc binding to laminin α5. After preincubation with anti-Lu/B-CAM antibodies, Lu-Fc was used in the binding assay on tissue sections. Antibody 87207 inhibited the binding of Lu-Fc to endogenous laminin α5.